Prosecutors had accused Noor Salman of obstructing justice and helping her husband, Omar Mateen, plan the 2016 attack that left 50 people, including Mateen, dead.
A federal judge denied the defense team's motion for a mistrial Monday. Noor Salman's lawyers had that argued prosecutors withheld key details, including Seddique Mateen's past connections to the FBI.
Prosecutors say Noor Salman assisted the 2016 rampage at Pulse nightclub and misled police. Her attorneys, however, say she was a "simple woman" who was abused by him and coerced by law enforcement.
In 2016, Noor Salman's husband opened fire at Pulse nightclub, killing 49 people and wounding dozens more. Salman is accused of aiding terrorism and obstructing justice.