Prosecutors had accused Noor Salman of obstructing justice and helping her husband, Omar Mateen, plan the 2016 attack that left 50 people, including Mateen, dead.
A federal judge denied the defense team's motion for a mistrial Monday. Noor Salman's lawyers had that argued prosecutors withheld key details, including Seddique Mateen's past connections to the FBI.
In 2016, Noor Salman's husband opened fire at Pulse nightclub, killing 49 people and wounding dozens more. Salman is accused of aiding terrorism and obstructing justice.
Hundreds gathered outside the nightclub Monday to remember the 49 people who were killed that night. Mourners have created a makeshift memorial there — and plans for a permanent one are underway.
"This must and will be a healing initiative," said owner Barbara Poma. It will commemorate the victims, survivors and rescue workers at the site of the deadliest mass shooting in modern U.S. history.
Noor Salman was arrested earlier this week and charged with providing material support to a terrorist and obstruction of justice. Her husband, Omar Mateen, killed 49 people at the Pulse club in June.
Charges against Noor Salman include "Aiding and Abetting by providing material support to a terrorist," Orlando's police chief said. The attack was the worst mass shooting in modern U.S. history.
Early Monday morning, a fire broke out at the Florida mosque where Omar Mateen worshipped. Police say the fire was "set intentionally," and they say surveillance footage shows someone at the site.
Omar Mateen did not have illegal drugs or alcohol in his system, according to an autopsy report released by the medical examiner in Orange County, Fla.
"We are disappointed that someone felt compelled to violate the privacy of our beloved Pulse Night Club and the sacred place it has now become," the club's owner said.