On Saturday, 25-year-old Ash Barty brought Australia its first singles home win in the Open since the 1978 edition. The final game for the men's singles will be played on Sunday.
Immigration Minister Alex Hawke said he canceled Djokovic's visa on "health and good order grounds, on the basis that it was in the public interest to do so."
The tennis star, who sought a COVID-19 vaccine exemption to play in the tournament, could face deportation again. The battle has sparked protests in the widely vaccinated country.
If he fails to have his visa cancellation overturned and gets deported, Novak Djokovic risks missing more than one Australian Open and could be barred for up to three years.
"We will work with everyone involved to facilitate testing as quickly as possible. There will be no matches at Melbourne Park on Thursday," Australian Open officials said in a statement.